Your partner for medical collagen

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Benefits of collagen

Due to its central role in cell adhesion and organ formation, collagen is an ideal substrate for cell culture and tissue engineering. Using an advanced technology for collagen purification and processing, our unique fibrous collagen retains the in vivo-like structure of insoluble collagen fibers with excellent proven biocompatibility and biodegradability.

Our collagen products provide natural signals for better attachment, growth, and differentiation, thereby supporting authentic cell performance and complex tissue formation.

COLLAGEN

COLLAGEN is the most abundant protein in mammals and can be found in many organs, e.g. tendons, ligaments, cartilage, blood vessels and dermis.

COLLAGEN TYPE I

COLLAGEN TYPE I is a major structural component of the extracellular matrix where it forms fibrils and fibers that build a scaffold for cell adhesion and the formation of connective tissues.

 

Unique carrier for cell analyses in their natural environment

The collagen cell carrier (CCC) represents a universal scaffold for adherent primary cells, cell lines and stem cells for in vitro and in vivo use in 2D and 3D cultures. The mechanical characteristics and the biocompatibility of this collagen type I carrier facilitate the establishment of co-cultures and the engineering of thin transferable tissue constructs, thereby offering new possibilities in the fields of cell culture techniques, tissue engineering, and regenerative medicine. As the CCC is self-supporting, the intact cell-scaffold complex can be transferred from the culture dish for in vitro or in vivo applications.

  • The cell-populated carrier can be treated directly with standard fixing reagents and is suitable for embedding in paraffin or preparation for cryostat microtomy to produce histological sections of the cell-matrix complex.
  • In phase contrast microscopy the CCC’s native collagen fibers hamper direct monitoring of flatly adhering cells. However, due to the low thickness (< 20 µm) and nearly no auto-fluorescence of the Collagen Cell Carrier, cells on top of the membrane can be directly stained with live cell dyes or other fluorescence markers and easily monitored even through the scaffold by fluorescence microscopy. For embedding onto a glass slide the intact cell-loaded carrier is simply pulled from the well bottom.
  • For cell analysis, cells can be lysed on the CCC and used directly for PCR or protein assays with only low contamination by collagen proteins.
  • The CCC withstands low temperatures and can be stored even in liquid nitrogen without any detrimental effects upon thawing.
  • The CCC is non-porous which allows the cultivation of different cell types on either side of the membrane. It is permeable for most soluble factors thus enabling tissue nutrition and paracrine cell-to-cell communication. It is therefore possible to perform tissue engineering at the air-liquid interphase and to study the transport of factors from one cell population to the other.

Read more information on CCC's product features and its in vivo use in clinical applications

The suitability and superior performance of the CCC for cell and tissue culture use has been thoroughly assessed in collaborations with a multitude of academic partners who proofed the qualification of our scaffolds in studies for various indications, such as cardiac insufficiency, age related macula degeneration or urethra repair.

 

Cell Culture in 3D

Our soluble collagen in R&D quality is suited to polymerize hydrogels for cell culture in 3D or to generate thin coatings on surfaces for 2D cell culture. Applications include tissue engineering of skin, bones, vascular system or other organs. This advanced culture matrix enables the analysis of cell migration in and on a natural surface, e.g. during metastasis, wound healing and development.

Viscofan BioEngineering‘s Viscolma® fibrillar collagen suspension with its putty-like consistency is the ideal material for coating of medical devices. It is also suitable for individual research applications like the development of 3D scaffolds.

Viscofan BioEngineering‘s Collagen Type I BioTubes have been used as scaffold material for cell seeding in bioreactor development.

 

Skin model

Adequate in vitro-generated human skin equivalents that resemble the cellular and structural properties of native skin represent an attractive alternative to animal models for biological infection studies. Viscofan BioEngineering‘s CCC has been used to develop a three-dimensional human skin equivalent as a model for the investigation of percutaneous worm invasion [Jannasch M et al., 2015]. The biological infection study has demonstrated that the skin equivalent provides a novel, excellent in-vitro model to analyze helminth invasion that can replace in vivo preparations.

 

Contact us to discuss your application needs.

primary cells


stem cells


cell lines


endothelial cells


cardiomyocytes


myocytes


hepatocytes


chondrocytes


osteoblasts


keratinocytes


glial cells



CCC Characteristics


  • self-supporting
  • minimal auto-fluorescence
  • low thickness (20 µm)
  • resistance to chemicals
  • good permeability for soluble factors
  • non-porous for cells (in absence of collagenases)
  • suitable for insertion into insert/transwell structures
  • cell compatibility (>30 primary cell types tested)
  • non-degradable in cell culture (in absence of collagenases)

CCC In Vitro Applications


  • universal culture of adherent cells
  • transferable 2D & 3D cultures
  • co-cultures from both sides
  • cell culture at liquid-air interface
  • applicable in bioreactors
  • fluorescence microscopy
  • histological analyses
  • protein or PCR assays